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The package was subsequently upgraded. In 2008, a new version (2.0) was built with the assistance of Héloïse Lavigne. Five additional functions were included to assist the design of perturbation experiments to investigate ocean acidification and other functions were revised in order to strictly follow the \"Guide to best practices for ocean CO2 measurements\" (Dickson et al., 2007). Seacarb uses equations mostly from the following sources:\n\n- Dickson A. G., Sabine C. L. \u0026 Christian J. R., 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3:1-191.\n- DOE, 1994. Handbook of methods for the analysis of the various parameters of the carbon dioxide system in sea water; version 2. Dickson, A. G. and Goyet, C., editors. ORNL/CDIAC-74, 1994.\n- Frankignoulle M., 1994. A complete set of buffer factors for acid/base CO2 system in seawater. Journal of Marine Systems 5: 111-118.\n- Orr J. C., Epitalon J.-M., Dickson A. G. \u0026 Gattuso J.-P., 2018. Routine uncertainty propagation for the marine carbon dioxide system. Marine Chemistry 207:84-107.\n- Zeebe R. E. \u0026 Wolf-Gladrow D. A., 2001. CO2 in seawater: equilibrium, kinetics, isotopes. Amsterdam: Elsevier, 346 pp.\n\nA portion of the code has been adapted, with permission from the authors, from the Matlab files mentioned above. I am grateful to Richard Zeebe and Dieter Wolf-Gladrow for that. Héloïse Lavigne contributed a lot to seacarb in 2008 and 2009; this was critical to the launch of version 2.0 and subsequent updates. Portions of code and/or corrections have also been contributed by Jean-Marie Epitalon (2004, 2018), Bernard Gentili (2006), Karline Soetaert (2007) and Jim Orr (2007, 2010, 2018). Jean-Marie Epitalon considerably improved the code, leading to much faster calculations in version 3.0.\n\nOrr et al. (2015) assessed seacarb together with other packages which calculate the seawater carbonate chemistry. In 2018, option to calculate uncertainty propagation was added (Orr et al., 2018).\n\n- Orr J. C., Epitalon J.-M., Dickson A. G. \u0026 Gattuso J.-P., 2018. Routine uncertainty propagation for the marine carbon dioxide system. Marine Chemistry 207:84-107. http://dx.doi.org/10.1016/j.marchem.2018.10.006\n- Orr J. C., Epitalon J.-M. \u0026 Gattuso J.-P., 2015. Comparison of ten packages that compute ocean carbonate chemistry. Biogeosciences 12:1483-1510. http://www.biogeosciences.net/12/1483/2015/\n\nThis program is provided free under the GNU General Public License (GNU GPL). It will be improved using the comments that I will receive. If you are new to R, please check the manuals and FAQs available on the R-project web site to get information on how to install R and the seacarb package on your system. Please only report and comment on seacarb, not on general problems related to R.\n\nBriefly, after installing R and if you have an Internet connection, here is the simplest way to install seacarb:\n\n- Launch R\n- To install seacarb (to be done only once), type the following command: install.packages(\"seacarb\")\n- To load the seacarb package into memory in order to use it (to be done each time R is launched), type the following command: library(seacarb)\n\nThe seacarb package can be downloaded from the Comprehensive R Archive Network (CRAN; see link below). The documentation is included in the package and is accessible using standard R commands. Please give due credit to the publications mentioned above and cite seacarb as follows:\n\nGattuso J.-P., Epitalon J.-M., Lavigne H. \u0026 Orr J., 2021. seacarb: seawater carbonate chemistry. 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